Human Anti-HIV-1 gp160 Recombinant Antibody (clone PGT128) (CAT#: PABL-179)
Recombinant Human Antibody (PGT128) is capable of binding to HIV-1 gp160, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HIV-1 gp160 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-HIV-1 gp160 mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
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Figure 1 High-mannose patch antibodies were tested for competition with sCD4 on isolate JR-FL E168K/N192A Env displayed on the surface of 293T cells. The CD4 binding site antibody 12A12 was included as a positive control and the V3-specific antibody F425 was included as a negative control.
Sok, D., Pauthner, M., Briney, B., Lee, J. H., Saye-Francisco, K. L., Hsueh, J.,... & Bastidas, R. (2016). A prominent site of antibody vulnerability on HIV envelope incorporates a motif associated with CCR5 binding and its camouflaging glycans. Immunity, 45(1), 31-45.
Figure 2 the functional stability of all proteins was tested by monitoring binding to bNAbs, VRC01 (CD4bs specific), and PGT128 (V3 loop glycan specific) before and after heating.
Proteins were incubated at 60 or 100 °C for 1 h and cooled down to room temperature to monitor binding to bNAbs VRC01 (left panel) and PGT128 (right panel) immobilized on the surface of a CM5 chip. Biacore response units were plotted after incubation at different temperatures to compare stabilities. Cyclically permuted gp120 trimers are stable at 60 °C showing similar binding before and after heating to VRC01 and PGT128 antibodies. They retained detectable binding to these antibodies even after incubation at 100 °C for 1 h.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Species Reactivity
- HIV-1
- Clone
- PGT128
- Applications
- WB, ELISA, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- ENV; gp160; envelope glycoprotein; Envelope surface glycoprotein gp160; precursor; hypothetical protein; Envelope surface glycoprotein gp120; Envelope transmembrane domain
- Gene ID
- 155971
- UniProt ID
- P04578
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-382 | Human Anti-HIV-1 gp160 Recombinant Antibody (clone VRC08) | Neut, FuncS | Human IgG, κ |
| MRO-3710CQ | Human Anti-HIV-1 gp160 Recombinant Antibody (clone CH423) | ELISA | Human IgG1 |
| MRO-3712CQ | Human Anti-HIV-1 gp160 Recombinant Antibody (clone DZ) | ELISA, WB, Neut, FC | Human IgG1, λ |
| MRO-3713CQ | Human Anti-HIV-1 gp160 Recombinant Antibody (clone F172-D8 (F172-D8, D8)) | ELISA, WB, IF | Human IgG |
| MRO-3714CQ | Human Anti-HIV-1 gp160 Recombinant Antibody (clone A1) | ELISA | Human IgG1, κ |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABL-179, RRID: AB_3111634)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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